The inhibitory activity of PGE at d

The inhibitory activity of PGE at different concentrations (0.12,1.2, and 12 g/l), was evaluated using spore suspensions of B. cinerea,P. digitatum, and P. expansum. To assess the effect of PGE on theviability of the pathogens' conidia, 0.5 ml of conidial suspensions(2.103 conidia/ml) were transferred to 1.5 ml Eppendorf tubescontaining 0.5 ml of PGE solutions with double concentrations ofextract (2 ) in order to obtain the above desiredfinal concentrations(1 ). Tubes containing 0.5 ml of citric acid 2% were used asa control. The obtained mixtures containing approximately1000 conidia/ml were gently mixed and incubated at 22 C.After 20 h, tubes were vortexed and 100 ml of conidia suspensionswere transferred and uniformly distributed in Petri dishescontaining PDA amended with ampicillin and streptomycinsulphate (250 mg/l each). Dishes were incubated at 25 C andthe number of colony forming units (CFU) was recorded after3–4 days.

The inhibitory Activity of PGE at different concentrations (12:12,
1.2, and 12 G / L), was evaluated using Spore suspensions of B. cinerea,
P. digitatum, and P. expansum. To Assess the Effect of PGE on the
viability of the pathogens' conidia, 0.5 ml of Conidial suspensions
(2
?
103 conidia / ml) were transferred to 1.5 ml Eppendorf Tubes
containing 0.5 ml of PGE Solutions with double concentrations of
Extract (2?). in Order to obtain the above desired
Final concentrations
(1?). Tubes containing 0.5 ml of citric acid 2% were used as
a Control. The obtained mixtures containing approximately
1000 conidia / ml were gently mixed and incubated at 22? C.
After 20 H, Tubes were Vortexed and 100 ml of conidia suspensions
were transferred and uniformly Distributed in Petri dishes
containing PDA amended with ampicillin and streptomycin
sulphate (250. mg / l each). Dishes were incubated at 25? C and
the Number of Colony forming Units (CFU) was Recorded after
3-4 days.

The inhibitory activity of PGE at different, concentrations (0.121.2 and 12, g / L), was evaluated using spore suspensions of, cinerea B.P. Digitatum and, P. Expansum. To assess the effect of PGE on the.Viability of the pathogens", conidia 0.5 ml of conidial suspensions.(2.103 conidia / ml) were transferred to 1.5 ml Eppendorf tubes.Containing 0.5 ml of PGE solutions with double concentrations of.Extract (2) in order to obtain the above desired.Final concentrations.(1). Tubes containing 0.5 ml of citric acid 2% were used as.A control. The obtained mixtures containing approximately.1000 conidia / ml were gently mixed and incubated at 22 C.After 20 h tubes were, vortexed and 100 ml of conidia suspensions.Were transferred and uniformly distributed in Petri dishes.Containing PDA amended with ampicillin and streptomycin.Sulphate (250 mg / L each). Dishes were incubated at 25 C and.The number of colony forming units (CFU) was recorded after.3 - 4 days.