ความเที่ยง (precision) : เตรียมสารล

Precision (precision): prepare a standard solution containing CU noi Kerry Mitsubishi various concentrations. : 5, 10, 15 and 20 mg/L, respectively, and the injection of HPLC systems, access to 10 μ L ponmit. Make a one-day trial in 7 (intra-day), and then repeat the experiment between วนั 7 (inter-day) the results to calculate a value S.D R.S.D maen (accuracy), or demand: extracts prepared by weighing (crude extract) 10 mg weight adjust volume 10 mL with ethanol, for example, the volume of pet health trends 500 μ L. Put into the bottles measure 4 volume 5 mL 1 bottle fine volume with ethanol. Sections 2, 3 and 4 bottles fill the solution come to rest the bases Kerry Mitsubishi 5, CU noi volume 10 and 15 respectively, and then adjust the volume L μ with full volume with ethanol is 1 mL injection of HPLC systems, access to the NA to volume of 10 L μ. Do repeat the experiment and the results 3. Calculate the percentage return (% recovery).

The reliability (precision): Prepare a standard solution curcumin increases are concentrated in the 5, 10, 15 and 20 mg / L, respectively, leading to injection into the HPLC system at global Mia sqm 10 μL. The experiment is repeated on the same day seven replicates (intra-day) and the experiment repeated between Nacional 7 repeatedly (inter-day) the effectiveness goes calculation of the SD or RSD accuracy (accuracy): preparation. by weighing extracts (crude extract) weights 10 mg adjustable volume 10 mL with ethanol and pipette sample volume of 500 μL put into bottles volumetric size 5 mL of four bottles at one adjust volume by S. alcohol bottles on the second, third and fourth fill Set the Kerr solution. Takumi Patapsco volume amounted to 5, 10 and 15 μL, respectively, and adjust volume with ethanol until the volume is full 1 mL then Naํ to injection into the HPLC system the volume of 10 μL of the experiment is repeated three repeat it. The result was to Calculate the percentage of recoveries (% recovery).

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