, sugars (glucose and maltose) derived from the digestion process. Organic acids (including differential Rick and acetic) are analyzed with HPLC (High performance liquid chromatography) (Model, 1200 series. Agilent, technologyJapan) using Detector type RI (Refractive index) sugar and organic acids are separated by column type An ion exclusion (BIO, RAD. ,, Aminex HPX-87H USA) by the temperature set column 45 º C and use 4 mM H2SO4 is Mobile phase with flow 0.4 mL / minute.
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