วันนี้อากาศหนาวมาก

วันนี้อากาศหนาวมาก

ผลลัพธ์ (อังกฤษ) 1:

Very cold today.

ผลลัพธ์ (อังกฤษ) 2:

Today the weather is very cold

ผลลัพธ์ (อังกฤษ) 3:

Xylanase assay.A modification of the reducing sugar method described by Khan.(1980) was used for the assay of xylanase (EC 3. 2. 1. 8) activity.Oat spelt xylan (Fluka) was used as enzyme substrate. The.Reaction mixture contained 0.5 mL of 0.1% (w / V) substrate in 0.1 M.Sodium acetate buffer (pH 5.0) and 0.1 mL of cell - free culture.Supernatant. The mixture was incubated at 40oC in water bath with.Shaking for 30 min. The reducing sugar released was measured.Using, 3 5-Dinitrosalicylic acid (Miller 1952), and xylose as standard.The colour was developed by boiling in water bath for 5 min.Absorbance was read at 540 nm using spectrophotometer.(Spectronic, Genesys TMS USA). The released reducing sugar was.Expressed in units mL-1. One unit of activity was defined as amount.Of enzyme required to liberate 1 Thermal mol of xylose per minute under.The assay conditions. The xylanase activities of A. Niger ANL 301.Cultivated on agro-wastes (sawdust sugar, wheat, pulp and bran).And oat spelt xylan was monitored at 24-h intervals for a period of.168 h under submerged fermentation condition.