โดย A0 คือ ค่าการดูดกลืนแสงของ blan

By A0 is a suck up light of A1 is blank and suck up their substance.Extraction of the sample or standard substance3.4 Le yi ngasen cultureSE ลลม์ะ la reng Sai Yai (Caco-2 cell) from naresuan University independent rap directory using.Passage at 29-32-pixel in 75-T Ta raising flask (size 75 cm2), SE ลลท์ี่ใช in ตน้ started breeding.Cells/cm2 is equal to 4x105, and change the 2 days meal by meal se ลลท์ุก cell is complete.Medium with glucose in high quantities (DMEM) 10.0% heat-inactivated bovine fetal serum (FBS),Java is no amino acids (amino acid non-essential) 10 ml/liter, l-kluta (L-glutamine) has the 2.0.Mitsubishi, MOL/liter, mafo lali Hotel Xin b foe (amphotericin B) 0.5 mg/l, Jane Damai (gentamicin) 50.Mg/liter, mol/l HEPES 15 Mitsubishi lali, and sodium bicarbonate (sodium bicarbonate) Mitsubishi 44 lali.Mol/l.

By A0 is the absorbance of blank and A1 is the absorbance of substances
extracted samples or standards
3.4 Culture Village darn positive cells
cells time each cancer Laํ handlers issued Larger (Caco-2 cell) itself. The University of Phitsanulok The
passage at 29-32 Taํ ใs cells cultured in 75-T flask (size 75 cm2), the vehicle used by the cell to start raising tar were less
than 4x105 cells / cm2 and cultured cells change every night. 2 days of culture cells using the Complete
Medium. A high amount of glucose (DMEM) to 10.0% heat-inactivated fetal bovine serum (FBS),
amino acids not Haํ. A (non-essential amino acid) 10 ml / L, L - glutamine (L-glutamine) 2.0
mmol / L, Amtrak Four Territories ricin B (amphotericin B) 0.5 mg / L, Jane. Eyes Xin (gentamicin) 50
mg / l, HEPES 15 mmol / L and sodium bicarbonate (sodium bicarbonate) 44 ms
mol / liter.

By A0 is the absorption of blank A1 is and absorbance of
extract sample or standard compounds
3.4 culture. This cell
cill. Mmm. Cancer, LA to Sai's head. (Caco-2 cell) ไดรั found by Naresuan. Using
.Passage that 29-32 apply to culture cells in M. 75-T flask (size 75 cm2) by cells. This problem that in raising started the
as 4x105 cells / cm2. And the change of cultured cells. 2 every day by the cultured cells used is complete
medium with high amounts of glucose (DMEM) used 10.0% heat-inactivated fetal bovine serum (FBS), no hot water
amino acids are non-essential amino acid) 10 ml / L,, L - glutamine (L-glutamine) 2.0
mmol / L, amphotericin B (amphotericin B) 0.5 mg / L, the gentamycin (gentamicin). 50
mg / liter,HEPES 15 mmol / L and sodium bicarbonate (sodium bicarbonate) 44 mol / L)

.