​การพบการปนเปื้อนของเชื้อโรคในวัคซี

​Found contamination of germs in the current vaccine to demonstrate problems in the production process that are not standard. Therefore, screening for bacterial contamination during the production process and thus play an important role in quality control in the production of vaccine, which should be a quick method. Precision, accuracy and high sensitivity In this research aims to develop appropriate methods for the screening of Avian leukosis virus infection contamination, Avian adenovirus and Mycoplasma spp., which paid the fuel might be found in any vaccine that uses eggs to incubate in the manufacturing process and during the production process by designing specific Primer pairs for each fuel type, there is the PC output size r different and increase the quantity of bacterial DNA targets with multi-plex p c r and output from twarot by splitting the output array size of the PC by means of electrophoresis DNA agarose gel with marker. The test results showed that pairs of Primer (Myco-TakaraF2, Myco-TakaraR2) for Mycoplasma gallisepticum is a suitable Primer pairs to be developed: To researchers unable to perform advanced research to achieve the objectives of the research. If there is an opportunity to do a test of specific primer pair one pair in the reaction with DNA templates for more than 1 type and a specific study of how multi-plex p c r for further development.

The contamination of germs found in the current vaccine demonstrated problems in the manufacturing process is not standardized. Therefore, screening for contamination during the manufacturing process. Has an important role in quality control in the production of vaccines. This should be a way that is quick, precise, accurate. And high sensitivity This research aims to develop a suitable method for screening for contamination of pathogenic Avian leukosis virus, Avian adenovirus and Mycoplasma spp., Which is a bacteria that can be found in the vaccines used in the production of eggs. And during the production process By Design Primer pairs specific to each type of infection measures yield different PCR. And the DNA of bacteria targeted by multi-plex PCR. Then the output of the coming century. By separation of PCR products by gel agarose electrophoresis with DNA marker
test results showed that the couple Primer (Myco-TakaraF2, Myco-TakaraR2) for Mycoplasma gallisepticum Primer pairs that will be developed later. The directors were unable to carry out research to achieve the objectives of the research. If there is an opportunity to test the specificity of each primer pair in a reaction that is more than one type genomic DNA and determine the specificity of the multi-plex PCR. To develop further

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.The pathogen contamination of vaccines currently illustrates the problems in the production process is not standardized. Therefore, screening contaminated during the manufacturing process. It plays an important role in the quality control in the production of the vaccine.Precise, accurate and has high sensitivity. This research aims to develop a method for screening the contamination of infections, Avian leukosis virusAnd Avian adenovirus Mycoplasma spp.Which is the infections that may be found in the vaccine used eggs hatch in the manufacturing process, and during the manufacturing process by designing Primer pair that is specific to each type of infections to size yield different PCRThe product to be scanning. By separating the size of the output gel PCR method agarose electrophoresis compared with DNA marker
.The test results indicated that the couple, Primer (Myco-TakaraF2Myco-TakaraR2) for the Mycoplasma gallisepticum pairs Primer fitting is developed. The researchers were unable to conduct research into the stage of research. " If you have a chance to test specific pairs of primer them one by one.1 type and study the specific method of multiplex PCR for the further development.