Abstract: We investigated the short

Abstract: We investigated the short-termresponse of poplar roots to low and high nitrogenavailability in order to elucidate the mechanismsinvolved in nutrient acquisition. After 28 days offertilization with low versus high ammoniumnitrate, an increase in aboveground biomass wasobserved accompanied by a decrease in rootbiomass, reducing the root: shoot ratio after 28days. These changes in biomass allocation wereaccompanied by changes in root architectureand altered gene expression. The geneexpression response was evaluated after 7 daysusing a custom cDNA micrarray followingtransfer to low and high nitrogen supply. Wefound that 56 sequences were differentiallyexpressed in poplar roots. Many of these 56genes could be associated with putative roles indevelopment or response to biotic and abioticstress. A time course analysis of selected cellwall-related genes by RT-qPCR confirmed theexpression patterns obtained by microarray andalso showed the timing of this differentialresponse. Our results show that patterns oftranscript accumulation in roots of poplars areinfluenced by nitrogen supply, providingevidence of unique nitrogen-adaptativemechanisms.

Abstract: We Investigated The short-term
POPLAR Roots of Response to Low and High Nitrogen
availability in Order to elucidate The Mechanisms
Involved in nutrient Acquisition. After 28 days of
fertilization with Low Versus High Ammonium
nitrate, an increase in Aboveground Biomass was
observed accompanied by a decrease in root
Biomass, reducing The root: shoot ratio After 28
days. Changes in Biomass allocation These were
accompanied by Changes in root Architecture
and Altered Gene Expression. The Gene
Expression Response was evaluated After 7 days
using a Custom cDNA Micrarray following
Transfer to Low and High Nitrogen Supply. We
Found 56 sequences that were differentially
expressed in POPLAR Roots. Many of these 56
genes could be Associated with putative roles in
Response to biotic and abiotic Development or
Stress. A time course analysis of Selected Cell
Wall-Related genes by RT-qPCR confirmed The
Expression Patterns obtained by microarray and
also showed The Timing of this differential
Response. Our results show that Patterns of
Transcript accumulation in Roots of Poplars are
influenced by Nitrogen Supply, providing
Evidence of Nitrogen-adaptative Unique
Mechanisms.

Abstract: We investigated the short-term
response of poplar roots to low and high nitrogen
availability in order to elucidate. The mechanisms
involved in nutrient acquisition. After 28 days of
fertilization with low versus high ammonium
nitrate an,, Increase in aboveground biomass was
observed accompanied by a decrease in root
biomass reducing the, root: shoot ratio after. 28
days.These changes in biomass allocation were
accompanied by changes in root architecture
and altered gene expression. The gene
expression. Response was evaluated after 7 days
using a custom cDNA micrarray following
transfer to low and high nitrogen supply. We
found. That 56 sequences were differentially
expressed in poplar roots. Many of these 56
genes could be associated with putative. Roles in
.Development or response to biotic and abiotic
stress. A time course analysis of selected cell
wall-related genes by RT-qPCR. Confirmed the
expression patterns obtained by microarray and
also showed the timing of this differential
response. Our results. Show that patterns of
transcript accumulation in roots of poplars are
influenced by, nitrogen supply providing
.Evidence of unique nitrogen-adaptative
mechanisms.