Simultaneous saccharification and f

Simultaneous saccharification and fermentation studies were carried out using thermotolerant newlyisolated Kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheatstraw and sugarcane bagasse at 5–15% solid loading and 6–12 FPU g 1 substrate enzyme loading fordifferent time intervals 0–72 h at 42 C. Maximum ethanol achieved from rice straw, wheat straw andsugarcane bagasse with in-house crude cellulases from Aspergillus terreus was 23.23, 18.29 and17.91 mg mL 1 at 60 h with 10% solid load and 9 FPU g 1 substrate enzyme loading. Tween 80 1% (v/v)enhanced the ethanol yield by 8.39%, 9.26% and 8.14% in rice straw, wheat straw and sugarcane bagasse,respectively. External supplementation of b-glucosidase to the crude as well commercial cellulasesproduced maximum theoretical ethanol yield of 71.76%, 63.77%, 57.15% and 84.56%, 72.47%, 70.55% fromrice straw, wheat straw and sugarcane bagasse, respectively.

Simultaneous saccharification and fermentation Studies were carried out using thermotolerant Newly
isolated Kluyveromyces SP. with three different delignified lignocellulosic biomass viz. Rice Straw, Wheat
Straw and sugarcane Bagasse at 5-15% Solid loading and 6-12m FPU G? 1 Substrate enzyme loading for
different time intervals 0-72 h at 42? C. Maximum ethanol achieved from Rice Straw, Wheat Straw and
sugarcane Bagasse with in-House Crude cellulases from Aspergillus terreus was eleven twenty-three p.m., 6:29 p.m. and
17.91 mg mL? 1 at 60 h with 10% and 9 FPU load Solid G? 1 Substrate enzyme loading. Tween 80 1% (v / v)
ethanol yield by 8.39% The Enhanced, 26.09% and eight fourteen% in Rice Straw, Wheat Straw and sugarcane Bagasse,
respectively. External supplementation of B-glucosidase to The Commercial Crude As Well cellulases
produced Theoretical Maximum ethanol yield of 71.76%, 63.77%, 57.15% and 84.56%, 72.47%, 70.55% from
Rice Straw, Wheat Straw and sugarcane Bagasse, respectively.

Simultaneous saccharification and fermentation studies were carried out using thermotolerant newly
isolated Kluyveromyces. Sp. With three different delignified lignocellulosic biomass viz. Rice, straw wheat
straw and sugarcane bagasse at 5 - 15%. Solid loading and 6 - 12 FPU g  1 substrate enzyme loading for
different time intervals 0 - 72 h at 42  C. Maximum ethanol achieved. From, rice strawWheat straw and
sugarcane bagasse with in-house crude cellulases from Aspergillus terreus, was 23.23 18.29 and
17.91 mg. ML  1 at 60 h with 10% solid load and 9 FPU g  1 substrate enzyme loading. Tween 80 1% (V / V)
enhanced the ethanol yield by 8.39% 9.26% and, 8.14% in. Rice straw wheat straw, and, sugarcane bagasse
respectively.External supplementation of b-glucosidase to the crude as well commercial cellulases
produced maximum theoretical ethanol. Yield of 71.76% 63.77% 57.15% and,,,, 84.56% 72.47% 70.55% from
rice straw wheat straw, and, sugarcane bagasse respectively.