2.3. Video recordings and behavioural observation.In each experiment cameras were, placed 60 cm above the top of.Upper tier cages andrecordedbehaviour fromabove.Cameras, were.Placed about 15 hbefore recordings beganinorder to lethenshabituate.To the devices. All behaviours were scored by a single observer.Blind to the treatment groups. For scoring behaviours cages were,,Virtually divided into distinct areas (Fig. 1): PSA nest and others,,,Named "middle areas" (one area C in, 20-hen cages areas C and D.In 40-hen cages areas C,,, and D E F in 60-hen, cages). MoreoverEach area was subdivided into two parts to specify DB location: by.The feeder trough (space fromthe feeder trough - presented outside.The cages - to the closest perch) or in the central area (middle space.Between the two internal perches or between perch and dividing.Wire for 20-hen cages) (Fig. 1). The observation protocol is further.Detailed for each experiment.
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