เพาะเลี้ยงเชื้อ B.megaterium ใน cry

Cryo-tube B.megaterium infection in vitro and then taken to melt, and then placed in the flask size 150 ml and bring in curing device with shaking at any time by using the meal at 28 LB fuel curing ager° C 300 rpm then suck fuel from the flask 5 ml 150 ml size to fit in more then 50 ml flask size of Colchicine and thiocolchicine into it, and then every 3 days to mature the following 6 hours will bring, for example, to see the growth based on the value of 600 nm light suck. see product occurred by TLC and HPLC and microscopic examination.

Culture B.megaterium in cryo-tube, then the heat and add in a 150 ml flask and bring incubated in a shaking device at any time by using the agar LB ager incubated at 28 ° C 300 rpm. Then suck fuel from the flask size 150 ml to 5 ml into a flask size from 50 ml to fill Colchicine and thiocolchicine down then incubated further for 3 days every 6 hours to take samples to see growth by value. absorbance at 600 nm. See the product by TLC and HPLC and microscopic examination.

Culture B.Megaterium in cryo-tube then were melted, then put into flask size 150 ml and brought incubation in equipment with shaking all the time by using the agar. LB ager curing 28 ° C 300 rpm then suck infections from flask size 150 ml come 5 ml put into flask size 50 ml and fill.And the thiocolchicine down and were incubated in 3 day, every 6 hours. The examples to see growth by the absorption of light in 600 nm.See what products by TLC and HPLC and microscopic examination.