1. to prepare the meal from fertilizer plants, each 0.1 ml N:P: K and distilled water 1000 ml adjust the pH value to end with a base at KOH concentration 0.1 N, pH = 6.7. 2. Autoclave steam sterilization at 121° c for 15 minutes at temperature.3. after removing the seaweed fertilizer plant with a meal as 3 concentration: 0.5, 1, and 2 times by three concentrations, divided into 2 series that put the child and do not provide nutrients that the food sterilization, chopped measuring volume of 2 ml and in put in sets of 3 tubes.4. check quantity of algae colony in 0.1 ml. 5. suck the algae into a meal, seaweed meal combinations to adapt from the fertilizer plant, the amount of 20% from the amount of food, it's sucking 2 ml 0.4 ml each came to trial, the secondary nutrients feed algae that recipe to make algae with growth and control the light coming off and brought 5,000 cotton that has been covered with Cork, plastic, rubber band come.6. check the amount of algae every 7 days, manage cattle for a period of 3 weeks.
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