where, T = 2.303, N is the multiple dilutions (250), C is the protein concentration (g/mL) in an aqueous solution of protein before emulsion formation, Q is the volume fraction of the oil phase in the emulsion (assumed to be 0.25), A0 is the absorbance of the diluted emulsion after homogenization, and A30 is the absorbance of the emulsion after remaining stationary for 30 min.
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