Project title: inhibiting the growth of fungal Aspergillus sp. And Penicillium sp., On rubber pads. Using extracts essential oils from cinnamon, lemon and mixtures cinnamon with lime
username prepared by Ms. Nantaporn affiliated sand at No. 17
, Ms. Buck Master Eye dog prowess No. 19
, Ms. Lewis and Mary Det Si Suwan number. 24
students a year 5/3
Advisor: Professor Piya Sulphur Brahmans
important concepts and
current rubber production in Thailand accounts for a major manufacturer of nearly 80% in order to meet the needs of the world's natural rubber. The proportion accounted for 30% of total global production of natural rubber processing. (Direction, production, processing and export of natural rubber in Thailand, 2548), but because farmers have a problem with mold on the rubber sheet. The quality of the rubber sheet is reduced. The fungus found mainly Aspergillus sp. Penicillium sp., Causing problems on rubber pads down. This affected the economy of the rubber market (Deacon, 1997; Chew et al., 2001; Clausen and Yang, 2003)
, the study's research Subhash Mon Nan Hiran and Warissara favorite Sun Valley has. studies on fungi Asperigillus sp., Penicillium sp The results showed that the extract essential oils from cinnamon and lemon. Which is effective in inhibiting fungal Asperigillus sp. And Penicillium sp fungus which Asperigillus sp. Extracts of cinnamon, can inhibit the fungus can best Penicillium sp. Lemon extract is effective in inhibiting best. Which extracts essential oils from cinnamon and lemon has the ability to mold the two. Staff are so interested to study the extract of cinnamon, lemon and cinnamon mixture with lemon. To be used to inhibit fungal Asperigillus sp. And Penicillium sp. In a rubber sheet with a test of Supansa. Chan works with
the essential oils of cinnamon, lemon extract might be another alternative for those who are interested in using natural, safe things around. The fungi Asperigillus sp., and Penicilli. sp., on rubber pads
, so Staff is interested to study the inhibition of the fungus Aspergillus sp. and Penicillium sp., on rubber sheets using extracts of cinnamon essential oil. Lemon and cinnamon mixture with lemon
hypothetical
one. Extract solvent type may have different abilities to inhibit fungal
second. The ratio of the MIC and will have the ability to inhibit fungal different
objectives
: 1. To study the solvent for the extraction of essential oils from cinnamon, lemon peel and cinnamon mixture with lemon. The fungi Aspergillus sp. And Penicillium. SP.
2. To determine the MIC and MFC extract essential oils that can inhibit Aspergillus sp. and Penicillium sp. were the best
3. To determine the appropriate ratio of extracted essential oil with MIC and MFC minimum. the fungi Aspergillus sp. and Penicillium sp., on rubber sheets scope of the project first. Fungi Aspergillus sp. And Penicillium sp on a rubber sheet 2. Two types of aromatherapy essential oils of lemon 3. The solvent is water Ethnan two types of cholesterol 4. MIC and MFC of essential oils of lemon. Cinnamon and lemon mixture with cinnamon five. The fungi Aspergillus sp. And Penicillium sp on rubber pads to perform at one of the solvents used for the extraction of essential oils from cinnamon, lemon peel and cinnamon mixture with lemon. The fungi Aspergillus sp. And Penicillium SP. 1.1 How to extract essential oils from cinnamon and lemon variables: Solvent refined water, ethanol variables: the ability to inhibit the fungus Aspergillus sp. and Penicillium sp. . Control: The concentration of oil Warped Hall Lama, the oil temperature. , Duration, type of plant one. Take a lemon rinse thoroughly. Then dried at 80 ° C for 10 HR 2. Remove lemon peel, cut to a length of about 0.5 cm 3. lemon slice weighing 500 g each, then put into a round bottom flask. 2 L distilled water The maximum heat in the pot evaporates, about 24 HR 4. Do the step 3 with ethanol 5. Follow steps 1-4 with cinnamon 1.2. The test their ability to inhibit the fungus. How disc diffusion assay variables: lime, lemon, cinnamon, cinnamon sterilized distilled water, acetic acid, acetic acid, citric. Acetic acid, cinnamon Acetic acid, lemon, cinnamon variables: the ability to inhibit the fungus Aspergillus sp. and Penicillium sp. Control: This type of agar., Temperature, Time 1. Preparation for the test fungus 2. Bring broth to a sterile PDA. Pour into Petri dishes 20 mL volumetric wait until the agar solidifies 3. Remove mold 0.1 mL volume drops on the agar. Spread over 15 minutes left 4. Place the paper, cut into 0.5 cm diameter circle 5. extracts, essential oils of lemon drops in on 30 uL drive down the middle of the agar 6. Repeat 3 times incubated at 30 ° C for 7 days in Autoprocessor control incubator record clear zone of fungus 7. Follow steps 1 to 6 oils with extracts of cinnamon, lemon, cinnamon, lemon water activists killed. Infection acetic acid, acetic acid, citric. Acetic acid, cinnamon Acetic acid, cinnamon, lemon Respectively the second study, the MIC and MFC extract essential oils that can inhibit Aspergillus sp. and Penicillium sp. were the best variables: the concentration of essential oils variables: MIC and MFC Control: mold. The concentration and volume of the fungus Aspergillus sp. and Penicillium sp. The volume of oil temperature, duration of fungus 1. Aromatherapy fungi Aspergillus sp. and Penicillium sp. The best of the first to find value. MIC and MFC below 1.1 extracted the best from the first to the concentration of 1000, 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 3.9 and 1.95 mg / ml, respectively 1.2 substances comfort. Put in a hole at 1-10 wells per 500 μL, respectively, 1.3, 11 holes, put Ketoconezoie 500 μL of holes as positive control, and the 12th hole, put a hole that was Broth 1000 μL Negative Control 1.4 Preparation fungus Aspergillus sp. and. Penicillium sp., the concentration was 106 cfu / ml, then put into the holes of the 12 hole 1.5 is left at room temperature for two days, repeated 3 times, then save the results for low concentrations of most of the substances town. Limited to fungi (MIC) Aspe.
การแปล กรุณารอสักครู่..