rain tissue and serum enzyme-linked

rain tissue and serum enzyme-linked immunosorbent
assay (ELISA)
At 0.5, 1, 3, and 7 days after MCAO, rats (n = 5–6 per group
per time point) were deeply anesthetized with 10% chloral hydrate,
and blood samples were collected from the abdominal
aorta. The brains were removed and immediately dissected on
ice. The cerebral hemisphere ipsilateral to the MCAO was dissected
out and stored at −80°C. Subsequently, brain samples
were homogenized in PBS buffer, the homogenate was centrifuged
for 45 minutes at 9,727 × g at 4°C, and the supernatant
was collected. Concentrations of brain lysate and serum NT3,
as well as brain levels of growth-associated protein 43 (GAP-
43), were quantified using a commercially available NT3 and
GAP-43 ELISA kit (Ever Systems Biology Laboratory, Sacramento,
CA, USA). Protein concentrations were determined
using the bicinchoninic acid method

ผลลัพธ์ (อังกฤษ) 2:

serum enzyme-linked immunosorbent Rain and tissue
assay (ELISA)
At 0.5, 1, 3, and 7 days after MCAO, rats (n = 5-6 per Group
Point per time) were passed with 10% Chloral hydrate deeply,
and Blood samples. Collected were from the abdominal
aorta. Brains were immediately removed and dissected on the
Ice. The cerebral hemisphere ipsilateral to the MCAO was dissected
out and stored at -80 ° C. Subsequently, Brain samples
were homogenized in PBS buffer, the homogenate was Centrifuged
for 45 minutes at 9727 × G at 4 ° C, and the supernatant
was Collected. Brain lysate and serum concentrations of NT3,
as well as Brain levels of growth-associated protein 43 (GAP-
43), were quantified using a Commercially available NT3 and
GAP-43 ELISA Kit (Ever Systems Biology Laboratory, Sacramento,
CA, USA). . Protein concentrations were determined
using the method Bicinchoninic acid.