Infectious myonecrosis virus (IMNV) is a recently observed shrimp virus, which threats the cultured Litopenaeus vannamei and can cause huge economic loss in shrimp farming industry. The specific aim of this study was to develop a new sensitive real-time PCR method for the specific detection of shrimp IMNV. A real-time PCR assay with a pair of primers to specifically amplify a 101 bp IMNV cDNA fragment and a corresponding TaqMan probe was developed, which shown to be specific for IMNV without cross reaction with DNA samples prepared from four other shrimp viruses including white spot syndrome virus(WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), and infectious hypodermal and haematopoietic virus (IHHNV). The method could detect as low as one single copy of IMNV plasmid cDNA.Shrimp industry.Export revenues, with several thousand million baht of Thailand. But now farmers began experiencing problems from an epidemic in shrimp pond. Make a lot of revenue loss, country Thailand. The cause of the epidemic in shrimp, mainly caused by a virus, which can be verified by using the PCR technique makes it possible to prevent the spread of the disease, Nick.
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