Carotenoid extraction was conducted

Carotenoid extraction was conducted according to Rodriguez-
Amaya (2001), with all stages being developed in a temperaturecontrolled
environment, with reduced lighting and antioxidant
addition (butylated hydroxytoluene-BHT). Aliquots of buriti pulp
(1.0 g) were used for carotenoid extraction with cold acetone
through shaking and vacuum filtration, three consecutive times.
The extracts were then transferred to the petroleum ether and
washed with distilled water in a separatory funnel. The ether
extract was read in a V-630 UV–Vis spectrophotometer (Jasco,
Tokyo, Japan), with absorbance between 350 and 700 nm. For the
calculation, the carotenoid absorbance at peak wavelength and
the molar absorption coefficient of b-carotene in petroleum ether
(2592) were used.

ผลลัพธ์ (อังกฤษ) 2:

Carotenoid extraction was Conducted according to Rodriguez
Amaya (in 2001), with all Stages being developed in a Temperaturecontrolled
Environment, with reduced Lighting and Antioxidant
addition (butylated hydroxytoluene-BHT). Aliquots of buriti pulp
(1.0 G) were Used for carotenoid extraction with cold Acetone
Through shaking and Vacuum FILTRATION, Three consecutive Times.
The extracts were then Transferred to The Petroleum ether and
washed with distilled Water in a Separatory Funnel. The ether
Extract was read in a V-630 UV-Vis SPECTROPHOTOMETER (Jasco,
Tokyo, Japan), with absorbance between 350 and 700 NM. For The
calculation, The carotenoid absorbance at wavelength Peak and
The Molar absorption coefficient of B-carotene in Petroleum ether
(2592nd) were Used.