The synthetic enzyme cellulase
enzyme cellulase synthesis mechanism. Born on the ribosomes in the cytoplasm cytosine
mRNA template by a specific act copy of each gene cellulase gene. It will then pass through
the cell membrane into the walls before. Or released to the outside immediately (Lehninger, 1977)
is an adaptive enzyme cellulase which are synthesized using cellulose as a carbon source
, but will be less synthetic. Or not yet in a source of glucose synthesis (Mandels and Reese, 1957)
to control the synthesis of the enzyme cellulase to be related to the induction and catabolite
repression. Which may be independent of each other Or occur together If the synthetic enzyme cellulase is controlled
by induction cellulose will not be induced to synthesize the enzyme. Because you can not enter the
cells. So it is with the enzyme in the synthesis of small quantities can cause degradation yields
soluble. And can enter cells to induce the synthesis of the enzyme cellulase to
Cyclic adenosine 3 ', 5' monophosphate (cAMP) is an important process
because catabolite repression in the culture. With the use of glucose as an energy source makes cAMP
intracellular concentration is low. Therefore, the synthetic enzyme that is inhibited by energy sources other
proteins. The mechanism for the biosynthesis enzyme cellulase may be caused by glucose acts
as end product repression as Eriksson (1978) studied the biosynthesis enzymes
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cellulase in Sporotrichum pulverulentum found that the glucose concentration 0:25. -0.5% will cause
catabolite repression of endoglucanase and exoglucanase
in the year 1980 Goksoyr and Eriksen finds that the biosynthesis enzyme cellulase
done two ways to control the biochemistry and genetics. The regulation of genes associated
with the induction, repression and catabolite repression cellulase is inducible enzyme
with the inductor include cellulose, cellobiose, thiocellulose, sophorose and chemicals such as
palmitate and acetate ester of disaccharide etc. Studies in microbes Found that the
enzyme synthesis is induced by cellulose in the form of soluble and can enter cells
have. Cellulase enzymes normally produced by a microorganism. But sometimes extracellular enzyme found in the wall
or cell membrane with. The study found that Trichoderma reesei QM9414 can produce
cellulase enzymes excreted by the cell into large quantities of food at low concentrations cellobiose. Or
that when cultured in conditions that induce the production. β-glucosidase Enzymes are ejected when
the cell autolysis.
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