DPPH assay is how to analyze the ability of antioxidant (antioxidant), which is, reagent 2 2-diphenyl-1-picrylhydrazyl. A method that is convenient, quick, easy to analyze, accuracy and precision, high
. PrincipleDPPH is stable radical in methanol (methanol) solution is purple, which absorb light at the wave length 515-517 nm. (nm)
.How, when DPPH - reaction with a compound with antioxidant activity. The color of the solution will turn yellow, purple By comparison with the antimicrobial conservative Mu independent used as standard.The intensity of the purple solution is reduced.
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