In this study, optimization of the

In this study, optimization of the surface culture conditions of C. militaris for cordycepin production was carried out. Most of the cordycepin synthesized by C. militaris existed in the culture medium. For the medium condition, the mixture of peptone and yeast extract in which the yeast extract ratio was greater than 75% was suitable, and the optimal carbon/nitrogen ratio (w/w) was 2/1 when glucose and the mixture of peptone and yeast extract (P/Y = 1/3) were used as the carbon and nitrogen sources, respectively. As for the medium depth, the lower medium depth leads to a higher productivity in the 1.8–5.3 cm medium depth

646 M. Masuda et al. / Enzyme and Microbial Technology 39 (2006) 641–646


range, because the utilization efficiency of the biofilm decreases with an increase in the medium depth over this experimental range. The maximum concentration of cordycepin obtained in this study was 640 mg/l, which was considerably high compared to previous reports.

Besides the nucleic acid-related compounds, polysaccharides with weight-average molecular weights of ca. 20,000 and 250,000 were obtained from the culture medium and the mycelia, respectively. In addition, D-mannitol was obtained from only the mycelia. Studies on other physiologically active com-pounds are future projects.

ผลลัพธ์ (อังกฤษ) 2:

In this study, optimization of the surface culture conditions of C. militaris for cordycepin production was carried out. Most of the cordycepin synthesized by C. militaris existed in the culture medium. For the medium condition, the mixture of peptone and yeast extract in which the yeast extract ratio was greater than 75% was suitable, and the optimal carbon / nitrogen ratio (w / w) was 2/1 when glucose and the mixture of peptone and. yeast extract (P / Y = 1/3) were used as the carbon and nitrogen sources, respectively. As for The Medium depth, The Lower Medium depth Leads to a Higher Productivity in The Medium 1.8 to 5.3 cm depth 646 M. Masuda et AL. / Enzyme and Microbial Technology 39 (2006) 641-646 Range, Because The efficiency of utilization decreases with an increase in biofilm The Medium The depth over this Experimental Range. The Maximum Concentration of cordycepin obtained in this Study was 640 mg / L, which was compared to High Considerably Previous reports. Besides The nucleic acid-Related compounds, polysaccharides with molecular weights of ca. Weight-Average 20,000 and 250,000 were obtained from the culture medium and the mycelia, respectively. In addition, D-mannitol was obtained from only the mycelia. Studies on other physiologically active com-pounds are future projects.