Method details
In this silica/alginate two-step immobilization procedure, we use Transwell as a support for immobilized protoplasts, which has three unique properties. (1) The tissue culture-treated membrane has a good affinity for the plant cell; thus, protoplasts can spread evenly and form a very thin layer. (2) There are more choices for membrane pore size, depending on corresponding applications. (3) It is very convenient to change or collect the solution without mechanically disturbing the protoplasts. We hope this simple and effective silica sol–gel/alginate two-step immobilization of protoplasts in
Transwell will have great potential for applications in genetic transformation, metabolites production and migration assays.