The study about the response of the tissue reaction in the teeth of both the histology and molecular biology. With emphasis on the development of guidelines for the treatment of tissue. Only by studying the response of tissue to chemicals and drugs, dental care is also a priority for research to develop pulp regeneration to create tissue in newly tooth is dead last. Such studies are usually performed in the teeth of animals. Or by techniques of cell culture in the laboratory , by the way, we have made teeth derived from tooth to tooth volunteers in 30 people in nutrients teeth under the oven sterile at a temperature of 37 ° C in extreme conditions. 5% CO2 for 7 days without changing the medium every two days after seven days, we have the teeth that fed into formalin before to study the morphology of cells, odontoblast and pulp fibroblast tooth through. fixation and decalcified with acid to make 10% nitric acid for one week until the teeth are soft and washed with 1X PBS and have it embedded in paraffin block was cut 5 micron vertical longitudinal section for staining. H & E analysis by observing the shape. And the number of cells, odontoblast, fibroblast comparison between dental control.
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