DPPH radical scavenging activityThe

DPPH radical scavenging activity
The antioxidant activity was determined using 2,2-diphenyl-l-picrylhydrazyl (DPPH) [7] .
Samples for testing were dissolved in absolute ethanol or distilled water to obtain the highest
concentration of 200 μg/ml. Each sample was further diluted for at least 4 concentrations (twofold
dilutions). Each concentration was tested in triplicate. A portion of the sample solution (100
μl) was mixed with an equal volume of 6 x 10-5 M DPPH (in absolute ethanol) and allowed to
stand at room temperature for 30 minutes. The absorbance (A) was then measured at 520 nm.
BHT (butylated hydroxytoluene), a well known synthetic antioxidant, was used as a positive
standard. The scavenging activity of the samples is the ability to reduce the color intensity of
DPPH. Inhibition (%) was calculated using the following equation.
% inhibition = [(A control – A sample)/A control] x 100
All tests were determined in triplicate. EC50 values (effective concentration of sample
required to scavenge DPPH radical by 50%) was calculated from the Prism program.

ผลลัพธ์ (แอฟริกา) 1:

DPPH radikale blaas aktiwiteit
Die antioksidant aktiwiteit is bepaal deur gebruik 2,2 difeniel-L-Picrylhydrazyl (DPPH) [7].
Monsters vir toetsing is in water opgelos Absolute etanol of gedistilleerde om die hoogste te verkry
konsentrasie van 200 g / ml. Elke monster is verder verdun vir ten minste 4 konsentrasies (tweeledig
verdunnings). Elke konsentrasie is getoets in triplikaat. 'N Gedeelte van die monster Oplossing (100
Μl) is gemeng met 'n gelyke volume van 6 x 10-5 M DPPH (in absolute etanol) en toegelaat word om
vir 30 minute by kamertemperatuur staan. Die absorbansie (A) is dan gemeet teen 520 NM.
BHT (Butylhydroxyanisool hydroxytoluene), 'n bekende sintetiese anti-oksidant, is gebruik as 'n positiewe
standaard. Die blaas aktiwiteit van die monsters is die vermoë om die kleurintensiteit van Verminder
DPPH. Inhibisie (%) is bereken met behulp van die volgende vergelyking.
% Inhibisie = [(A beheer - 'n Monster) / A beheer] x 100
Alle toetse is vasgestel in triplikaat. EC50-waardes (effektiewe konsentrasie van die monster
wat nodig is om aas DPPH radikale met 50%) is saamgestel uit die Prism-program.