The composition of the culture medium, low cost and suitable conditions of cultivation of soybean meal. In the production of the enzyme protease. The bacteria B. subtilis is required for optimal conditions using mathematical methods and statistics. The Box-Behnken experimental design was designed to determine the appropriate level of the variable that K2HPO4, MgSO4 • 7H2O, pH, and time is a significant result in the production of the enzyme protease. When cultured on a diet consisting of high level K2HPO4 (-1) was 0.80 g, MgSO4 • 7H2O at a low level (-1) and pH level is 0.60 g (-1) was 7.0 in production. at 21 h, resulting in the production of the enzyme protease Most 50.13 Unit / ml, which is close to the value predicted by the statistical model 50.84 Unit / ml format used is appropriate that produce enzymes. Protease most Food recipes developed to keep production costs down by 41.49% compared to Nutrient Broth 0.8 g / 100 ml and soybeans, 2.4 g / 100 ml of the enzyme protease was 24.00 Unit / ml and 29.60 Unit /. ml, respectively, which is achieved by the use of low-cost source of carbon and nitrogen.
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