Sample of 4 g + water 40 ml into beakers
pH 7 with sodium hydroxide concentration of 2 M
enzyme alpha amylase 400 microliter
incubated 37 degrees for
sampling each time the centrifuge 1200 rpm long. 10 minutes
to bring the clear 0.25 ml + distilled water, 1.25 ml put in a tube
filling DNS reagent 1.5 ml
gag vitro boil water boil 5 minutes, to set at room temperature,
adding a solution of potassium the letter concentrated 40% volume 0.5 ml
lead. to measure light at a wavelength of 530 nm see if
compared with standard glucose curve.
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