Molecular Biology-Based Identification:The first proposed
molecular biology-based identification method was based
on PCR-restriction fragment length polymorphism (RFLP)
analysis of the gene encoding the OmpA protein. Molecular
detection using polymerase chain reaction (PCR) is possible
from skin rash biopsies, lymph node biopsies or
ethylenediaminetetraacetic acid (EDTA) blood.O.
tsutsugamushi can be demonstrated by standard and by
nested PCR. Realtime PCR assays are as sensitive as
standard PCR but are more rapid and can give quantitative