At present, breast tumor in dogs can be classified by the diagnosis by histopathology The development technique of immune no hiss grew up chemical to select tumor marker collected June chest breast of 1 samples Use of antibodies specific to vimentin for mesenchymal tumors and evaluation from the position and intensity of infection. Many Found that the sample is caught light brown in the cytoplasm (+ positive), which data from the sample used in the study Found that tumors such as a kind of tumor Mast cell tumor skin cancer of dogs Originated from the bone marrow and scattered in various connective tissue in the body, which can occur in the internal organs of their education OZAK faculty and found that the use of vimentin to diagnose the tumor mastcell (Mast cell tumor) positive 15% and from the study of Aydogan The 44.18% will stick positive color light (Weak +) for vimentin found indicators with specificity in cancer types other than as Transmissible venereal tumor the diagnosis Mast cell tumor or mastocytoma with this method has been used Tumor marker other kind Such as Ki-67 PCAN, etc. This study suggests that the diagnosis of breast tumor in dogs that can be done in several ways And one of them is technically immune no hiss grew up chemical Based on the difference of stem cell