1). Tune the spectrophotometer to t

1). Tune the spectrophotometer to the desired wavelength.
2). Load a cuvette with the solvent used in your sample
3). Place the cuvette in the spectrophotometer, and close it.
Then tare the spectrophotometer.
4). Once the spectrophotometer is tared, then remove the solvent cuvette, and load a
cuvette with your sample. Record the absorbance value that it gives you.
5). This procedure NEEDS to be repeated at EACH wavelength.

Absorbance is different for EVERY chemical at EVERY wavelength,
so every time you change one of them, the spectrophotometer will need to be tared.

ผลลัพธ์ (อังกฤษ) 2:

1). Tune to the desired wavelength spectrophotometer the.
2). Load a cuvette with the Solvent used in your sample
3). Place the cuvette in the spectrophotometer, and close it.
Then Tare the spectrophotometer.
4). Once the spectrophotometer is Tared, then Solvent Remove the cuvette, and Load a
cuvette with your sample. Record the absorbance that it gives You Value.
5). NEEDS to be repeated this procedure at EACH wavelength. Absorbance is different for at EVERY EVERY Chemical wavelength, so every time one of them Change You, the spectrophotometer Will Need to be Tared.