เรียนรู้ได้เร็วและอดทนต่องานหนักได้

เรียนรู้ได้เร็วและอดทนต่องานหนักได้

ผลลัพธ์ (อังกฤษ) 1:

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ผลลัพธ์ (อังกฤษ) 2:

Assay of agarase activity. Cultures were then centrifuged to remove bacteria, the supernatant was treated with 0.2 vol. 2.5% (w/v) Cetrimide and the resulting precipitate was removed by centrifuging. The supernatant was treated with 2 vol. acetone at 4oC for 30 min. The precipitate was collected by centrifuging and redissolved in 5mM KH2PO4/Na2HPO4 buffer (pH 7.4). The activity of this crude extracellular agarase was assayed with a solution of agarose (Sigma; 0.2% w/v) in 5mM KH2PO4/Na2HPO4 buffer (pH 7.4). The agarase preparation (0.5 mL) was mixed with 0.5 mL agarose solution and 2 mL 10mM KH2PO4/Na2HPO4 buffer (pH 7.4) and incubated at 29oC for 1 h. The reaction was stopped by adding 1 mL copper reagent and the reducing sugars were measured colorimetrically by the method of Dygert et al. (1965) except that any precipitate of undegraded agarose was removed by centrifuging (2600g) for 15 min. Blanks of substrate with no enzyme and enzyme with no substrate were treated in the same way. One unit of agarase activity was defined as the release of reducing groups equivalent to 1μmol D-galactose in 1 h at 29oC, pH 7.4

ผลลัพธ์ (อังกฤษ) 3:

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