This article presents a strategy to get the ingredients in small quantities from herbal extracts that are complicated by a combination of simple. Sephadex column LH-20 co and co mass used to prepare liquid (LC) method. tenacigenin, Tenacigenin b and 17 β-tenacigenin Certified lack any significant chromophores from marsdenia tenacissima chloroform extracts of m. tenacissima is initially to a Sephadex column, lh-20, two-output fraction from 20 mg of small molecular weight. 3.0 mg 2.0 mg tenacigenin tenacigenin b and 3.0 mg 17 β-b tenacigenin excluded through a successful advance preparation-LC. Hire a mass fraction of storage as in contrast to the active isolation system applied previously, correct detergent system through one at a time, as the economy with high comfort. The substance has been all three above 90%, as defined by: Lc alongside rayekrachai check light (ELSD) and their structure is specified, the nmr spectrum. © The Royal Society is 2014.
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