Individual 250 ml Erlenmeyer flasks

Individual 250 ml Erlenmeyer flasks, each containing 50 ml of
PRP-amended broth (Louw and Webley, 1959) were inoculated
with 3 ml of 20% glycerol suspension of each of the 16 isolates
(approximate 10 8 cfu ml ?1 ), plugged with cotton wool and the
flask walls covered with aluminum foil and incubated on a
rotary shaker at 250 rpm at 30 8C in the dark. After 12 days, the
suspension from each flask was centrifuged for 30 min at
5000 ? g. The supernatant fluid was then filtered through
sterile millipore membranes (pore size 0.22 mm, Millipore
Corporation, MA, USA) to remove cell debris. The filter-
sterilized cell-free culture broth was collected in sterile
McCartney vials and allowed to settle for 15 min at room
temperature for the determination of pH. Control consisted of
sterilenon-inoculated medium. Water-soluble P was analyzed
bythecolorimetricprocedureofMurphyandRiley(1962)using
molybdophosphoric acid blue complex. There were eight
independent replicate flasks for each isolate. The pH drop and
the amount of released soluble P were taken as an indicator of
the efficiency of selected isolates.ฉันรักการแปล

ผลลัพธ์ (อังกฤษ) 1:

Individual 250 ml Erlenmeyer flasks, each containing 50 ml ofPRP-amended broth (Louw and Webley, 1959) were inoculatedwith 3 ml of 20% glycerol suspension of each of the 16 isolates(approximate 10 8 cfu ml ?1 ), plugged with cotton wool and theflask walls covered with aluminum foil and incubated on arotary shaker at 250 rpm at 30 8C in the dark. After 12 days, thesuspension from each flask was centrifuged for 30 min at5000 ? g. The supernatant fluid was then filtered throughsterile millipore membranes (pore size 0.22 mm, MilliporeCorporation, MA, USA) to remove cell debris. The filter-sterilized cell-free culture broth was collected in sterileMcCartney vials and allowed to settle for 15 min at roomtemperature for the determination of pH. Control consisted ofsterilenon-inoculated medium. Water-soluble P was analyzedbythecolorimetricprocedureofMurphyandRiley(1962)usingmolybdophosphoric acid blue complex. There were eightindependent replicate flasks for each isolate. The pH drop andthe amount of released soluble P were taken as an indicator ofThe efficiency of selected isolates. I love the translation.

ผลลัพธ์ (อังกฤษ) 2:

Individual 250 ml Erlenmeyer flasks, each containing 50 ml of
PRP-amended broth (Louw and Webley, the 1959th) were inoculated
with 3 ml of 20% glycerol suspension of each of the 16 isolates
(approximate 10 8 cfu ml? 1), Plugged with. Cotton Wool and the
Flask Walls covered with aluminum foil and incubated on a
Rotary shaker at 250 RPM at 30 8C in the Dark. After 12 days, the
suspension from each Flask was Centrifuged for 30 min at
5000? G. The supernatant Fluid was then. Through filtered
sterile Millipore membranes (Pore Size 0.22 mm, Millipore
Corporation, MA, USA) to Remove Cell debris. The filtering
Cell-free Culture broth was sterilized Collected in sterile
vials and allowed to settle for McCartney 15 min at Room
Temperature for the. determination of pH. Control consisted of
Sterilenon-inoculated Medium. Water-soluble P was analyzed
BythecolorimetricprocedureofMurphyandRiley (1,962) using
Molybdophosphoric acid blue Complex. There were Eight
Independent replicate flasks for each isolate. The pH Drop and
the amount of Released soluble P were taken. as an Indicator of
the efficiency of selected isolates. I love the translation.

ผลลัพธ์ (อังกฤษ) 3:

Individual 250 ml Erlenmeyer flasks each containing, 50 ml of
PRP-amended broth (Louw and Webley 1959), were inoculated
with 3 ml. Of 20% glycerol suspension of each of the 16 isolates
(approximate 10 8 CFU ML? 1), plugged with cotton wool and the
flask. Walls covered with aluminum foil and incubated on a
rotary shaker at 250 rpm at 30 8C in the dark. After, 12 days the
.Suspension from each flask was centrifuged for 30 min at
5000? G. The supernatant fluid was then filtered through
sterile. Millipore membranes (pore size 0.22 mm Millipore
Corporation,,, MA USA) to remove cell debris. The filter -
sterilized cell-free. Culture broth was collected in sterile
McCartney vials and allowed to settle for 15 min at room
temperature for the determination. Of pH.Control consisted of
sterilenon-inoculated medium. Water-soluble P was analyzed
bythecolorimetricprocedureofMurphyandRiley (1962) using
Molybdophosphoric. Acid blue complex. There were eight
independent replicate flasks for each isolate. The pH drop and
the amount of released. Soluble P were taken as an indicator of
the efficiency of selected isolates. I love translation.