. The FRAP reagent comprised of2.5

. The FRAP reagent comprised of
2.5 ml of 10 mM 2,4,6-tripyridyl-s-triazine (TPTZ, Sigma-Aldrich,
St. Louis, MO) in 40 mM HCl, 2.5 ml of 20 mM iron(III) chloride
(FeCl3, Sigma-Aldrich, St. Louis, MO) and 25 ml of 0.3 M acetate
buffer pH 3.6. Briefly, 20 ll of extract was added with 200 ll of
FRAP reagent into the 96-well microplate and the homogenate
was incubated at 37 C for 4 min. Blank was prepared by replacing
0.1 ml of polyphenol extract with 0.1 ml of dH2O. The absorbance
value of the blue-colored complex formed was measured against
a blank at 593 nm using microplate spectrophotometer. The experiment
was performed similarly in the preparation of standard
curve by using 20 ll of different concentration of iron(II) sulphate
heptahydrate (FeSO47H2O, Sigma-Aldrich, St. Louis, MO). FRAP
value was determined from the standard curve (100–1000 lM;
R2 = 0.9993) and expressed as mmol Fe(II) g
1 FW.

ผลลัพธ์ (แอฟริกา) 1:

. Die FRAP reagens bestaan uit
2,5 ml van 10 mm 2,4,6-triasien-Tripyridyl-s (TPTZ, Sigma-Aldrich,
St Louis, MO) in 40mm HCl, 2,5 ml van 20 mm Yster (III) chloried
(. FeCl3, Sigma-Aldrich, St Louis, MO) en 25 ml van 0,3 M asetaat
buffer pH 3.6. Kortliks, 20 ll van Extract is bygevoeg met 200 ll van
die 96-goed Microplate In FRAP reagens en die homogenate
was vir 4 minute geïnkubeer by 37 C. Leeg is opgestel deur die vervanging van
0,1 ml polyphenol Uittreksel met 0.1 ml DH2O. Die absorbansie
van die blou-gekleurde WAARDE Kompleks gevorm is gemeet teen
'n leë behulp Microplate spektrofotometer by 593 NM. Die eksperiment
was so presteer in die voorbereiding van standaard
kurwe deur die gebruik van 20 ll van verskillende konsentrasie van yster (II) sulfaat
heptahydrate (FeSO4? 7H2O, Sigma-Aldrich, St Louis, MO). FRAP
waarde bepaal van die standaardkromme (100-1000 LM;
R2 = 0,9993) en uitgedruk as mmol Fe (II) G 1 FW?.