Remove laccase Enzyme (200-800 mgL-1) in aqueous solution of sodium Alginate solutions (1.0, 1.5, 2.0, 3.0 and 4.0% w/v), respectively, and then shake The mixture together, and that was dropped into a syringe by CaCl2 solution (1-4% w/v), then Shaking the next hr 1 Washed with CaCl2., 0.5% (w/v) to DI water, followed by twice, respectively, then it would have. Immobilized laccase beads and kept in DI water at 4 °C.
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