KERTr cells cultured 3T3 A31 cell lines and then tested with Acetic acid at a concentration of 0.0625% -8% were then incubated for 15 minutes, rinsed with PBS 2 rounds, then put the food on it. By incubated for 24 hours and check the result by the appearance of cells under a microscope, inverted (Inverted Microscope) and measure the survival rate of the cells by MTT assay.
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