The results Find the right temperature for the primer to bind to genomic DNA annealing step is in the range 37 to 39 degrees Celsius, depending on the type of prime polymers. And the concentration of MgCl2 appropriate that ranged from 2.5 to 4.0 mM (Ostrowska, 1998) as a cofactor MgCl2 to help the work of the Taq polymerase enzyme to the DNA to be longer. So, if the concentration of MgCl2 too few to make work less Taq polymerase enzyme. Increase productivity with greater specificity, resulting in a number of fragments less. However, the high concentration of the enzyme Taq polymerase MgCl2 are more viable. Thus, the DNA was not more specific. In addition, the genomic DNA is diluted 10 times (6.6 ng) to yield the PCR may be due to that. The extracted DNA was contamination inhibitor of the enzyme. When diluted, the contamination and are less inhibited. In the process of extracting DNA, not RNA enzyme Rnase A lead contamination in DNA extracted RNA, which may have to scramble to catch Mg2 +, making the performance of the Taq polymerase enzyme reduced.
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