2.4. Biological treatmentThe basidi

2.4. Biological treatment
The basidiomycete fungi species used in this study for the biological treatment of OMW, namely P. sajor caju and P. chrysos- porium (Burdsall 38388) were obtained from UNESP (São Paulo State University, Brazil) and from BCCMTM/MUCL Culture Collec- tion (Belgium), respectively. P. sajor caju and P. chrysosporium were cultured at 25 and 30 °C, respectively, in a medium containing 20 g L 1 of malt extract, 1 g L 1 of peptone and 16 g L 1 of agar. After a growth period, the mycelium of both species was preserved at 4 °C, during 5 d, in the culture medium, to keep it hydrated and with nutrients.
Before the biological treatment of OMW, the mycelia of P. sajor caju and of P. chrysosporium were grown in a liquid medium containing 20 g L 1 of malt extract and 1 g L 1 of peptone, at 25 °C and 120 7 10 rpm. Afterwards, the mycelia was collected filtering the culture media, through a sterilized gaze and, were kept in sterilized plastic containers at 4 °C, until the biological treatment of OMW.
The biological treatment of OMW samples was started by adding the ligninolytic fungi P. sajor caju and P. chrysosporium (5.0 7 0.1 g) individually to 190 ml of pre-diluted and treated OMW (by the photodegradation process as previously described) in glass beakers. The amount of fungi biomass added to each beaker, was based on a previous study from Ferreira et al. (2008). According to Jaouani et al. (2003), the following additives were added to the OMW replicates before the biological treatment: 1.0 g L 1 of potassium dihydrogen phosphate, 0.405 g L 1 of dia- mmonium tartrate dibasic and 0.05 g L 1 of yeast extract. Three replicates per photodegration pre-treatment and fungus species and three replicas with no photodegradation pre-treatment were inoculated with each fungus species. This was performed in ster- ilized Erlenmeyer flasks covered up with sterile hydrophobic cot- ton and gauze, and then incubated at 25 °C and 120710 rpm, for 21 d. Throughout the incubation period, aliquots of 5 ml of the treated effluent were withdrawn from each treatment at different moments (0, 3, 7, 10, 14, 17, 21 d) in order to monitor pH and ab- sorbance at 270 and 465 nm. Before the biological treatment and after 21 d of incubation with fungi, chemical characterization (COD and TPC) and ecotoxicological evaluation of OMW samples was also performed.

ผลลัพธ์ (อังกฤษ) 2:

2.4. Biological Treatment
The Basidiomycete fungi species used in this Study for the Treatment of Biological OMW, namely P. sajor caju and P. Chrysos- Porium (Burdsall 38388) were obtained from UNESP (São Paulo State University, Brazil) and from BCCMTM / MUCL Culture. Collec- tion (Belgium), respectively. P. sajor caju and P. chrysosporium were cultured at 25 and 30 ° C, respectively, in a medium containing 20 g L 1 of malt extract, 1 g L 1 of peptone and 16 g L 1 of agar. After a growth period, the mycelium of both species was preserved at 4 ° C, during 5 D, in the Culture Medium, to Keep it hydrated and with nutrients.
Before the Biological Treatment of OMW, the mycelia of P. sajor and of caju. P. chrysosporium were grown in a liquid medium containing 20 g L 1 of malt extract and 1 g L 1 of peptone, at 25 ° C and 120 7 10 rpm. Afterwards, the mycelia was filtering the Collected Culture Media, Through a gaze and sterilized, were kept at 4 ° C in sterilized Plastic Containers, until the Biological Treatment of OMW.
The Biological Treatment of samples was OMW Started by adding the Ligninolytic fungi P. sajor caju and P. chrysosporium (5.0 7 0.1 g) individually to 190 ml of pre-diluted and treated OMW (by the photodegradation process as previously described) in glass beakers. The amount of fungi biomass added to each beaker, was based on a previous study from Ferreira et al. (2008). According to Jaouani et al. (2003), the following additives were added to the OMW replicates before the biological treatment: 1.0 g L 1 of potassium dihydrogen phosphate, 0.405 g L 1 of dia- mmonium tartrate dibasic and 0.05 g L 1 of yeast extract. Three replicates per photodegration pre-treatment and fungus species and three replicas with no photodegradation pre-treatment were inoculated with each fungus species. This was performed in ster- ilized Erlenmeyer flasks covered up with sterile hydrophobic cot- ton and gauze, and then incubated at 25 ° C and 120710 rpm, for 21 d. Throughout the incubation period, aliquots of 5 ml of the treated effluent were withdrawn from each treatment at different moments (0, 3, 7, 10, 14, 17, 21 d) in order to monitor pH and ab- sorbance at 270 and 465. nm. Before the biological treatment and after 21 d of incubation with fungi, chemical characterization (COD and TPC) and ecotoxicological evaluation of OMW samples was also performed.

ผลลัพธ์ (อังกฤษ) 3:

2.4. Biological treatment.The basidiomycete fungi species used in this study for the biological treatment, of OMW namely P. Sajor caju and P. Chrysos -. Porium (Burdsall 38388) were obtained from UNESP (S the o Paulo State University Brazil), and from BCCMTM / MUCL Culture Collec -. Tion (Belgium), respectively. P. Sajor caju and P. Chrysosporium were cultured at 25 and 30 ° C respectively in a medium,,, Containing 20 g L 1 of, malt extract 1 g L 1 of peptone and 16 g L 1 of agar. After a growth period the mycelium, of both. Species was preserved at 4 ° C during D, 5, the culture, in medium to keep it hydrated and with nutrients.Before the biological treatment, of OMW the mycelia of P. Sajor caju and of P. Chrysosporium were grown in a liquid medium. Containing 20 g L 1 of malt extract and 1 g L 1, of peptone at 25 ° C and 120 7 10 rpm. Afterwards the mycelia, was collected. Filtering the, culture media through a sterilized gaze and were kept, in sterilized plastic containers at 4 ° C until the,, Biological treatment of OMW.The biological treatment of OMW samples was started by adding the ligninolytic fungi P. Sajor caju and P. Chrysosporium. (5.0 7 0.1 g) individually to 190 ml of pre-diluted and treated OMW (by the photodegradation process as previously described). In glass beakers. The amount of fungi biomass added to, each beaker was based on a previous study from Ferreira et al. (2008).? According to Jaouani et al. (2003), the following additives were added to the OMW replicates before the biological treatment: 1.0 g. L 1 of potassium dihydrogen phosphate 0.405 g, L 1 of dia - mmonium tartrate dibasic and 0.05 g L 1 of yeast extract. Three. Replicates per photodegration pre-treatment and fungus species and three replicas with no photodegradation pre-treatment. Were inoculated with each fungus species. This was performed in ster - ilized Erlenmeyer flasks covered up with sterile hydrophobic. Cot - ton and gauze and then, incubated at 25 ° C and 120710 rpm for 21, D. Throughout the, incubation period aliquots of 5 ml. Of the treated effluent were withdrawn from each treatment at different moments (0 3 7,,,,,, 10 14 17 21 d) in order to. Monitor pH and ab - sorbance at 270 and 465 nm. Before the biological treatment and after 21 d of incubation, with fungi. Chemical characterization (COD and TPC) and ecotoxicological evaluation of OMW samples was also performed.