Researchers collected saliva samples from the substance, all of the volunteers who keep the 6 examples of saliva before and after using mouthwash.By collecting saliva sample Cup holders 2 minutes then bring to fling with a fling at 3000 × g for a period of 2 minutes, keep clear of approximately 450 µL volume aqueous solution and perchloric acid into 50 µL volume to prevent changes to the nature of the substance acetaldehyde and protection condition of enzyme.
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