The rhizosphere soil possesses a Large and
diverse population of microorganisms
mainly the bacteria. Bacteria in the
rhizosphere influence Plant growth because
they affect soil Chemical properties and
INTERACT with Plant Roots, where the influence
Can be beneficial, Neutral, or deleterious
(Russell1981, Morita the 2000th, Sakaietal. The 2004th).
The microbes in the rhizosphere soil have
the Property. to compose the Complex
Substances depending on the Plant exudates
(one thousand nine hundred ninety-four Kimura, et al Rovira., 1,974th, Watt et
al., 2006b). In few decades a Large Number
of bacteria from the rhizosphere soil have
been isolated and identified among which
the common species of Pseudomonas,
Azospirillum, Azotobacter, Klebsiella,
Enterobacter, Alcaligens, Arthobacter,
Burkholderia, Bacillus and Serratia have
reported (Kloepper et al.,. 1 989; Okon and
Labandera-Gonzalez, one thousand nine hundred and ninety-four; Glick, the 1,995th).
ISSN: from 2319 to 7,706 Volume 3 Number 9 (2,014th) PP. 993-998
Http://www.ijcmas.com
Ke Ywo RDS
Indole Acetic
acid,
Rhizosphere,.
Oryza
sativum,
Biofertiliser
The rhizosphere soil is source for High Diversity of microorganisms which Play a
Major role in the enhancement of the Plant. The microbe and Plant Interaction
Plant influences the growth. In the present Study 9 different Rice rhizosphere soil
samples were Collected from different districts of Kolar, Hoskote, Ramnagar and
Mysore. Isolations were done on nutrient agar by Media Serial dilutions and
morphological variation with different Colonies were selected from each dilution. A
total of 53 isolates were differentiated and maintained as Pure Cultures on nutrient
agar. Biochemical and morphological characterization was carried out for all the
individual isolates. Both Gram positive cocci and bacilli along with Gram negative
bacilli Gram positive and also branched Colonies probably actinomycetes were
isolated in the present Investigation. A total of 57 Gram positive bacilli, 6 Gram
negative bacilli, Gram positive cocci and 47 actinomycetes were isolated Six of the
total samples used in the current Study. 53 isolates were screened for the IAA
and Production 6 isolates proved to Produce IAA ranging from 0.535 - 14.05μg / ml.
Further the organisms Will be screened for Production of growth promoting
Substances which Can be used as Natural Biofertilisers in the enhancement of the
yield of. Rice.
Int.J.Curr.Microbiol.App.Sci (the 2,014th) 3 (9) nine hundred ninety-three to nine hundred and ninety-eight
994
Rice (Oryza sativum) is one of the important
source of Calorie, it is a staple Cereal to be
consumed in Large Quantity throughout. the
World thus it is been Called as Queen among
the Cereals (Aadil Abbas et al., 2011). Rice
has been considered as one of the Major
Crops Cotton after wheat and throughout the
World and it has been cultivated on Area of
2590 thousand hectares with a Production of
5720 thousand ton (Muhammad asim et al.,
2008th). It provides Half of total dietary
carbohydrate, especially in Asian countries
and it is Suitable diet for more than Three
billion people, supplying 50-80% of their
Daily Calorie intake (khush, 2005). The
genus Oryza is Distributed in Tropical and
Sub Tropical Regions of the World.
The bacteria isolated from rhizosphere soils,
have proved to be beneficial to the Plants by
directly having an Effect on nitrogen
Fixation (Han et al., 2005th), Solubilization of
nutrients. (Rodriguez Fraga and the 1,999th),
Production of growth hormones, Aminocyclopropane- 1-
1-carboxylate (ACC)
deaminase (Correa et al., the 2,004th) and
Indirectly by antagonizing pathogenic fungi
by the Production of siderophores, -1,3-
glucanase,. antibiotics, fluorescent pigments
and cyanide (Pal et al., 2001).
IAA producing microorganisms are Known
to promote root elongation and Plant growth
(Patten and Glick 2002). IAA-Production by
root associated bacteria is a Major
Plant growth mechanism of this Promotion
has important implication for the
Development of the root System under the
influence of rhizosphere microbes (Anton
Hartmann et al., Two thousand and nine). IAA-biosynthesis of
the Plant growth promoting Rhizobacterium,
Azospirillum Brasilense was shown to be
induced in Rhizoplane colonizing bacteria
(Rothballer et al., In 2005).
The Objective of this present Study was to
isolate and Identify the Diversity of bacteria
from the rhizosphere of Nine. different
rhizosphere soil from Oryza sativum Plants
Grown in South Karnataka and to Check ITS
ability to Produce IAA.
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