Because the method previously used was stained with silver. Now, do not do it. So I'm not sure that this time you will be using pcr sequencing or fagment.
Because the original method used is dyed with silver color, which right now does not work, so I'm not sure that this time you will use the fagment method of sequencing or PCR.
because I had used the same method is to dye with silver color, now is not, and then, so I did not make sure that you will be using the methods in this PCR fagment sequencing?