Early blight of tomato, which is ma

Early blight of tomato, which is mainly caused by Alternaria solani, is an important, globally distributed
disease. We previously isolated an endophytic bacterium (BT4) from healthy tomatoes that has high antifungal
activity against A. solani. Industrial applications of antagonistic bacteria require the optimization
of culture conditions for growth and antifungal activity. Thus, in this study, the optimum medium composition
and culture conditions for BT4 were determined using the ‘‘one factor at a time’’ method and an
orthogonal design. Optimal cell growth and maximum antifungal activity were obtained at 30 C, pH 7.0,
with an inoculation proportion of 2% and incubation for 48 h in 12 g/L yeast extract, 10 g/L soluble starch
and 7 g/L CaCl2 in the culture medium. In addition, we compared the antifungal activity of BT4 with
iprodione, a contact fungicide that is used on a variety of plants affected by fungal diseases. The results
showed that BT4 had antifungal effects that were better than iprodione.

ผลลัพธ์ (อังกฤษ) 2:

Early blight of Tomato, which is mainly caused by Alternaria solani, is an important, Globally Distributed
disease. An endophytic bacterium we isolated previously (BT4) from Healthy tomatoes that has antifungal High
Activity against A. solani. Industrial Applications of antagonistic bacteria Require the Optimization
of Culture conditions for growth and antifungal Activity. Thus, in this Study, the Optimum Medium composition
and Culture for BT4 conditions were determined using the '' one factor at a time '' method and an
orthogonal Design. Optimal Cell growth and maximum antifungal Activity were obtained at 30? C, pH 7.0,
with an inoculation proportion of 2% and Incubation for 48 H in 12 G / L yeast Extract, 10 G / L soluble starch
and 7 G / L CaCl2 in. the culture medium. In addition, we compared the antifungal Activity of BT4 with
Iprodione, a fungicide that is used on a Contact Variety of Plants affected by fungal diseases. The results
Showed that had BT4 antifungal effects that were better than Iprodione.

ผลลัพธ์ (อังกฤษ) 3:

Early blight of tomato which is, mainly caused by Alternaria solani is important globally, an, distributed
disease. We. Previously isolated an endophytic bacterium (BT4) from healthy tomatoes that has high antifungal
activity against A. Solani.? Industrial applications of antagonistic bacteria require the optimization
of culture conditions for growth and antifungal. Activity. Thus in this study,,The optimum medium composition
and culture conditions for BT4 were determined using the '' one factor at a time method. '' And an
orthogonal design. Optimal cell growth and maximum antifungal activity were obtained at 30,  C pH 7.0
with, an inoculation. Proportion of 2% and incubation for 48 h in 12 g / L, yeast extract 10 g / L soluble starch
and 7 g / L CaCl2 in the culture, medium. In, additionWe compared the antifungal activity of BT4 with
iprodione a contact, fungicide that is used on a variety of plants affected. By fungal diseases. The results
showed that BT4 had antifungal effects that were better than iprodione.
.