3.4.4 fermented in bottles (Erlenmeyer flask) trial size 250 mL that resembles the arms protrude like to maintain the system, as the system is closed off with a Butyl rubber bottle cap section brachial side protrude is closed with the Rubber septum, and shot or sampling. Aseptic syringe to maintain state of the oxygen bottle is always devoid of this meal packed into bottles of fuel it will be steamed at 121 ºC into sterile 15 minutes before they stop oxygen in bottles with nitrogen gas to the anaerobic conditions continued to grow this type of bacteria, all experiments in this study, 35 ºC temperature fermentation, and will use the rotational speed at 100 rpm do the experiment 3.
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