icrobial counts, pH and temperature of liquid feed are shown in<br>Table 2. A sampling location × treatment interaction was observed for<br>LAB. Counts of LAB were lower in troughs of the 10kg/t BA treatment<br>than in troughs of all other treatments, but LAB counts in the mixing<br>tank were not influenced by BA inclusion rate. A tendency for a sampling location × treatment interaction was observed for Enterobacteriaceae counts. In liquid feed from the 2.5kg/t BA treatment,<br>counts of Enterobacteriaceae were higher in the trough than in the<br>mixing tank; however, counts in the mixing tank and trough were similar at all other BA inclusion rates. There was also a sampling location × treatment interaction for pH. In the mixing tank, the pH of the<br>10kg/t BA treatment was lower than the 0kg/t BA and 2.5kg/t BA<br>treatments, whereas in the trough, the pH of 0kg/t BA was lower than<br>for all other treatments. There were no sampling location × treatment<br>interactions for E. coli, yeast, or mould counts or feed temperature.<br>The main effect of treatment must also be considered. In the mixing<br>tank, counts of LAB, E. coli, yeast and mould and liquid feed temperature were similar for all treatments. Counts of Enterobacteriaceae in the<br>2.5kg/t BA treatment tended to be lower than those in the 0kg/t BA and<br>5kg/t BA treatments (P=0.06) but the same as the 10kg/t BA treatment<br>(P>0.05). The pH of the 10kg/t BA treatment in the mixing tank was<br>lower than all other treatments (P
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