It may be because the conditions are not appropriate and thus, bacteria in the sample could not flourish. E.g. Enterococci are frequently found in the frozen food and prosperity well at temperatures of 45 degrees Celsius, which has led to trials, curing at a temperature of 37 degrees Celsius, thus causing the infection can not flourish. Or is because of Enterococci. Endure alkali (pH = 9.6) in which the mussels are acids so it makes Enterococci can not flourish.
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