Then add the sodium carbonate concentration 7 by weight per volume (% w / V) number 1.25 mL mixture and adjust the final volume is 3 mL. With distilled water and shake, and to set at room temperature in the dark for 1 hours 30 minutes and then to the absorbed light at wavelengths, 760 nm With UV-VIS Spectrophotometer by testing samples each 3 times (triplicate) lead absorbance to build standard curve between concentration. Of gallic acid and absorb light.
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