Diets rich in fruits and vegetables

Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48h at 1 and 30µM, with or without phorbol ester treatment at 24h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1µM for 24h, with lipopolysaccharide added at 5h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly) phenols significantly altered cytokine release from Jurkat cells (P

ผลลัพธ์ (อังกฤษ) 1:

Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48h at 1 and 30µM, with or without phorbol ester treatment at 24h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1µM for 24h, with lipopolysaccharide added at 5h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly) phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferonγ induced protein 10by primary lymphocytes was significantly reduced following treatment with 1µM isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of proinflammatory mediators in people at risk of chronic inflammation.

ผลลัพธ์ (อังกฤษ) 3:

Diets rich in fruits and vegetables (FV), which contain (poly), phenols protect against age-related in fl ammation and chronic. Diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about. The relative potency of different (poly) phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly phenols.) And six (poly) phenol mixtures for effects on pro-in fl ammatory cytokine release by Jurkat T-lymphocytes. Test compounds were. Incubated with Jurkat cells for 48h at 1 and tracks, 30 M with or without phorbol ester treatment at 24h to induce cytokine, release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0 experience 2 and 1 tracks, M for 24h. With lipopolysaccharide added at 5h. Cytokine release, was measured and generation of H2O2 by test compounds was determined. To assess any potential correlations with cytokine release. A number of (poly) phenols signi fi cantly altered cytokine release. From Jurkat cells (P < 0 experience 05), but H2O2 generation did not correlate with cytokine release. Resveratrol isorhamnetin curcumin,,,, Vanillic acid and speci fi C (poly) phenol mixtures reduced pro-in fl ammatory cytokine release, from T-lymphocytes and there was. Evidence for interaction between (poly) phenols to further modulate cytokine release. The release of interferon γ induced. Protein 10 by primary lymphocytes was signi fi cantly reduced following treatment with 1 could M isorhamnetin (P < 0 experience 05). These results. Suggest that (poly) phenols derived from onions turmeric red grapes,,, tea green and a ç AI berries may help reduce the release. Of proin fl ammatory mediators in people at risk of chronic in fl ammation.