2.3. Primer design and synthesis
The forward and reverse primers were designed according to the sequences of matrix protein (M) gene (Gen-Bank accession no. GQ452014.1) of PPRV Sungri-96 strain by using Fast PCR software. The designed primers (Forward M3F:5GGAGTGATTGAGGATAACGACC-3(169–190) and Reverse M3R:5-GCGTTAACAAGGACAGCGGAG-3(350–370) were validated by Oligo Analyzer 1.2 and synthesized commercially (Sigma Aldrich,Bangalore, India).