Make the cells more concentrated lead to bacteria centrifuge at 4000 x g for 4 minutes and granular cells together and floating in water, acetone, to get hormones, 1010-1011 cells/ml.
More concentrated bacterial cells, leading to the centrifuges. 3500 × g for 4 minutes and the cell pellet gathered and flotation peptone -1011 to 1010 cells / ml.
The cell concentration to the rotating cast more the bacteria that 3500 × g for 4 minutes and granule cells combine together and floating in the water, peptone. In order to 1010 - 1011 cells / ml.