Sex steroid and thyroid hormone lev

Sex steroid and thyroid hormone levels were determined in serum
samples by direct competitive enzyme linked immunoassays (ELISA)
using a polyclonal rabbit anti-T antibody, a monoclonal mouse anti-T4
antibody or an anti-rabbit IgG, all of them coated onto the wells of 96
microwell plates (T and T4, Diagnostics Biochem Canada Inc., ON,
Canada E2, Diagnostics Automation Inc., CA, USA). All the reagents
and samples were brought to room temperature (18–25 °C) prior
to use. All the samples were analyzed in duplicate following the
supplier's instructions as previously reported for sea turtles
(Ikonomopoulou et al., 2008). The standard curves, the sensitivity
of the kits and the intra- and inter-assay coefficients of variability
are shown in Table 1. The concentrations of the hormones are
expressed in nmol T L−1, nmol T4 L−1 and pmol E2 L−1.

ผลลัพธ์ (อังกฤษ) 2:

Sex steroid and thyroid Hormone levels were determined in serum
samples by Direct competitive enzyme linked immunoassays (ELISA)
using a polyclonal Rabbit Anti-T antibody, a monoclonal Mouse Anti-T4
antibody or an Anti-Rabbit IgG, all of them coated onto the Wells. of 96
microwell plates (T and T4, Diagnostics Biochem Canada Inc., ON,
Canada E2, Diagnostics Automation Inc., CA, USA). All the reagents
and samples were brought to Room Temperature (18-25 ° C) prior
to use. Duplicate samples were analyzed in the following all the
Supplier's instructions as previously reported for Sea Turtles
(Ikonomopoulou et al., the 2008th). The standard curves, the sensitivity
of the Kits and the intra-assay coefficients of variability and Inter-
are shown in Table 1. The concentrations of the hormones are
expressed in nmol TL-1, L-1 nmol T4 and E2 pmol L-1. .