The crude sample mixture was subjec

The crude sample mixture was subjected to Preparative HPLC for separation into various
fractions. The mixture was separated in 26’by reversed phase HPLC on an Adsorbosphere
column-NH2, (250 x 4.6 mm column) using both isocratic and gradient elution with
acetonitrile/water and detected using (Waters ELSD 2420). In ELSD, the mobile phase is first
evaporated. Solid particles remaining from the sample are then carried in the form of a mist into
a cell where they are detected by a laser

ผลลัพธ์ (อังกฤษ) 2:

The mixture was subjected to preparative HPLC Sample Crude for Separation Into Various.
fractions. The mixture was separated in 26'by Reversed Phase HPLC on an Adsorbosphere.
column-NH2, (250 x 4.6 mm column) Isocratic and gradient elution using Both with.
acetonitrile / Water and detected using (Waters 2,420th ELSD). In ELSD, The mobile is First Phase.
evaporated. Solid particles remaining from The Sample are then carried in The Form of a mist Into.
a Cell Where they are detected by a Laser.