By A0 is the absorption of blank A1 is and absorbance of
extract sample or standard compounds
3.4 culture. This cell
cill. Mmm. Cancer, LA to Sai's head. (Caco-2 cell) ไดรั found by Naresuan. Using
.Passage that 29-32 apply to culture cells in M. 75-T flask (size 75 cm2) by cells. This problem that in raising started the
as 4x105 cells / cm2. And the change of cultured cells. 2 every day by the cultured cells used is complete
medium with high amounts of glucose (DMEM) used 10.0% heat-inactivated fetal bovine serum (FBS), no hot water
amino acids are non-essential amino acid) 10 ml / L,, L - glutamine (L-glutamine) 2.0
mmol / L, amphotericin B (amphotericin B) 0.5 mg / L, the gentamycin (gentamicin). 50
mg / liter,HEPES 15 mmol / L and sodium bicarbonate (sodium bicarbonate) 44 mol / L)
.
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