3.3. Microencapsulated viability of Bifidobacterium Bb-12 during
Storage
The viability of Bifidobacterium Bb-12 Microencapsulated by
Spray Drying with whey and stored at 4 C and remained Constant High
(> 9 Logcfu / G) for 12 weeks (Fig. 3). Similar results were obtained
by Brachkova et al. (The 2010th), Who Microencapsulated
Lactobacillus spp. in alginate. The viability of the microcapsules retained
on Silica gel remained throughout 6 months of Storage Stable
at 4 C.
However, Oliveira (2006) reported that the viability of B. lactis
followed by Spray Drying Microencapsulated by Coacervation with
pectin and casein Started to decline Within the. First 30 days of Storage
under Refrigeration, resulting in a decrease of 4.34 log Final
after 120 days. Zhao et al. (2008) noted a decrease from 1 to 1.5
log in the viability of L. acidophilus Microencapsulated by Spray
Drying with B-cyclodextrin and Acacia GUM during Storage for
8 weeks at 4 C.
In a Study by Chavarri et al. (The 2010th), the Survival of L. Gasseri and
B. Chitosan and alginate Microencapsulated with bifidum by extrusion
Decreased About 2 log after 28 days of Storage at 4 C. When
the capsules were also produced with the prebiotic quercetin, the
decrease in viability was even Greater, About 4.3 log after 11 days.
Brinques and Ayub. (2,011th) noted that decreases ranged from 1.95
to 6.73 Logcfu / G, depending on the encapsulating Agent used (alginate
or pectin), in the viability of L. plantarum Microencapsulated
during Storage for 38 days at 4 C.
The Final Challenge related to the. Development of probiotic
microcapsules is posed by their stability during Storage (Albertini
et al., 2,010). According to Corcoran et al. (2004), the stability of
probiotics is Greater Microencapsulated at low temperatures, such
as Temperature Refrigeration. Undoubtedly, the encapsulating
Agent also has a Direct Effect on the stability of the microcapsules.
Heat denaturation of whey Proteins influences emulsification characteristics
and thus Microencapsulating properties (Rosenberg and
Sheu, 1,996th). It was shown that Spray-Drying resulted in a denaturation
and Aggregation of whey Proteins (Millqvist-Fureby et al.,
2001), enhancing their adsorption at the interface and resulting
to the Formation of a thin, gel-liked layer (Kiokias et al. ., 2,007) .Thus,
whey has proven to be a good encapsulating Agent for maintaining
the viability of Bifidobacterium during Storage at 4 C.
3.4. Viability of the microcapsules in a Dairy Dessert
Fig. 4 shows the total viable Bifidobacterium Bb-12 Count of
Microencapsulated with whey in the Dairy Dessert stored at 4 C.
During the 6 weeks of Storage, there was a decrease of
0.05 ± 1/16 Logcfu / G; however, cell viability remained high
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